Announcements: Picture of the Month
Current Images from the DBG November 1st, 2013
Current Images from the DBG August 5th, 2013
The cells are porcine valve endothelial cells (VEC) and are stained for f-actin (white), cadherin (green), and DAPI (blue). Photo by Luke MacQueen. Credits to Hongyan Yuan for the patterns and to Andrew Capulli for cells and staining protocol.
Current Images from the DBG July 16th, 2013
The image shows harvested E18 stage Rat cortical neuronal cells cultured for 6 weeks in vitro prior to fixing and immunostaining via standard procedures for neurofilament (green), GFAP (Red) and DAPI (Blue), which label the intermediate filaments, astroglia and nucleus respectively. Photo by Eswar P. R. Iyer
Images from the DBG April 17th, 2013
Ventricular cardiomyocytes from neonatal rats can be cultured on substrates coated with extracellular matrix proteins anisotropically. These cardiomyocytes can grow according to spatial cues provided by proteins attached to a substrate. Soft-lithography allows for customizable, micron-scale protein patterns to be “stamped” onto a substrate. This allows for building tissues that more closely resemble their orzanization in vivo. This image shows cardiomyocytes aligned to 10 micron wide lines of fibronectin separated by 10 micron wide lines of fibronectin at a lower concentration.
Photo by Borna Dabiri
Images from the DBG November 14th, 2012
Using micro-contact printing techniques, extracellular matrix proteins can be deposited on substrates in custom shapes with micron scale resolution; by controlling ECM shape, pattern, size, etc we can recapitulate native tissue structure and alignment in our in vitro assays. Here is an aligned diamond tissue composed of porcine valve interstitial cells [blue: nucleus, red: vimentin, white: f-actin, green: alpha-SMA]. Photo by Andrew Capulli, Parker Research Group.
Picture of the Month – July 2012 July 18th, 2012
Purified primary harvest astrocytes from neonatal sprague-dawley rat stained for GFAP, astrocyte (red), Nuclear DNA (blue). Scale bar= 50um. Image by Chung Jong Yu, Parker Lab.
Picture of the Month – May 2012 May 22nd, 2012
“VICs and VECs” — Valve endothelial cells (VECs) are interspersed within an isotropic monolayer of valve interstitial cells (VICs). The monolayer is immunostained for VEC indicative CD31 (green) and VIC indicative alpha SMA (red) as well as dyed for filamentous actin (yellow) and nuclei (blue). Image by Andrew Capulli, Parker lab.
Picture of the Month – April 2012 April 6th, 2012
Isotropic cardiac myocyte monolayer stained for actin (red), beta-catenin (white), and nuclear DNA (blue). Image by Megan McCain, Parker lab.
Picture of the Month – February 2012 February 24th, 2012
Micropatterned mouse ventricular muscle cells assembled into an anisotropic tissue showing nuclei (pink) and organized sarcomeres (orange). This organization recapitulates features of the native architecture of cardiac tissue. Image by Anna Grosberg, Parker lab.
Picture of the Month – January 2012 January 12th, 2012
Micropatterned ventricular muscle cells from a mouse illustrated various stages of cell spreading on a soft hydrogel that mimics the microenvironment of cardiac tissue. The phases of the moon inspired the design. Image by Yvonne Aratyn and Sean Sheehy, Parker lab.