54. Agarwal A, Farouz Y, Nesmith AP, Deravi LF, McCain ML, Parker KK. Micropatterning Alginate Substrates for In Vitro Cardiovascular Muscle on a Chip. Adv. Funct. Mater. (Web): 19 MAR 2013.
53. Nawroth JC, Parker KK. Design standards for engineered tissues. Biotechnol Adv. 2012 Dec 23. doi:pii: S0734-9750(12)00198-X. 10.1016/j.biotechadv.2012.12.005. [Epub ahead of print] PubMed PMID: 23267860.
52. Kuo P, Lee H, Bray MA, Geisse NA, Huang YT, Adams WJ, Sheehy SP, Parker KK. Myocyte shape regulates lateral registry of sarcomeres and contractility. Am. J. Pathol. 2012; 181(6):2030-7.
51. Deravi LF, Su T, Paten JA, Ruberti, JW, Bertoldi K, Parker KK. Differential Contributions of Conformation Extension and Domain Unfolding to Properties of Fibronectin Nanotexiles. Nano Letters. 2012; 12 (11):5587–5592.
50. Nawroth JC, Lee H, Feinberg AW, Ripplinger CM, McCain ML, Grosberg A, Dabiri JO, Parker KK. A tissue-engineered jellyfish with biomimetic propulsion. Nat Biotechnol. 2012;30;792-797.
49. Dabiri BE, Lee H, Parker KK. A potential role for integrin signaling in mechanoelectrical feedback. Prog Biophys Mol Biol. 2012; 110: 196–203.
48. Sheehy SP, Grosberg A, Parker KK. The contribution of cellular mechanotransduction to cardiomyocyte form and function. Biomech Model Mechanobiol. 2012 Jul 7. [Epub ahead of print]
47. McCain ML, Lee H, Aratyn-Schaus Y, Kléber AG, Parker KK. Cooperative coupling of cell-matrix and cell-cell adhesions in cardiac muscle. PNAS. 2012;109:9881-9886.
46. Feinberg AW, Alford PW, Jin H, Ripplinger CM, Werdich AA, Sheehy SP, Grosberg A, Parker KK. Controlling the contractile strength of engineered cardiac muscle by hierarchal tissue architecture. Biomaterials. 2012;33:5732-5741.
45. Beauchamp P, Desplantez T, McCain ML, Li W, Asimaki A, Rigoli G, Parker KK, Saffitz JE, Kleber AG. Electrical Coupling and Propagation in Engineered Ventricular Myocardium With Heterogeneous Expression of Connexin43. Circ Res. 2012;110:1445-1453.
44. Grosberg A, Nesmith AP, Goss JA, Brigham MD, McCain ML, Parker KK. Muscle on a chip: In vitro contractility assays for smooth and striated muscle. J Pharmacol Toxicol Methods. 2012;65:126-135.
43. Desplantez T, McCain ML, Beauchamp P, Rigoli G, Rothen-Rutishauser B, Parker KK, Kleber AG. Connexin43 ablation in foetal atrial myocytes decreases electrical coupling, partner connexins, and sodium current. Cardiovasc Res. 2012;94:58-65.
42. Shim J, Grosberg A, Nawroth JC, Parker KK, Bertoldi K. Modeling of cardiac muscle thin films: Pre-stretch, passive and active behavior. J. Biomech. 2012;45:832-841.
41. Sheehy SP, Parker KK. The Role of Mechanical Forces in Guiding Tissue Differentiation. In: Bernstein H, editor. Tissue Engineering in Regenerative Medicine. Springer; 2011:77-97.
40. Balachandran K, Alford PW, Wylie-Sears J, Goss JA, Grosberg A, Bischoff J, Aikawa E, Levine RA, Parker KK. Cyclic strain induces dual-mode endothelial-mesenchymal transformation of the cardiac valve. PNAS. 2011;108:19943-19948.
39. McCain ML, Desplantez T, Geisse NA, Rothen-Rutishauser B, Oberer H, Parker KK, Kleber AG. Cell-to-cell coupling in engineered pairs of rat ventricular cardiomyocytes: relation between Cx43 immunofluorescence and intercellular electrical conductance. Am J Physiol Heart Circ Physiol. 2012;302:H443-H450.
38. Mellado P, McIlwee HA, Badrossamay MR, Goss JA, Mahadevan L, Parker KK. A simple model for nanofiber formation by rotary jet-spinning. Appl Phys Lett. 2011;99:203107.
37. Grosberg A, Alford PW, McCain ML, Parker KK. Ensembles of engineered cardiac tissues for physiological and pharmacological study: Heart on a chip. Lab Chip. 2011;11(24):4165-4173.
36. Alford PW, Nesmith AP, Seywerd, JN, Grosberg A, Parker KK. Vascular smooth muscle contractility depends on cell shape. Integr. Biol. 2011;3(11):1063-1070.
35. Dvir T, Timko BP, Brigham MD, Naik SR, Karajanagi SS, Levy O, Jin H, Parker KK, Langer R, Kohane DS. Nanowired three-dimensional cardiac patches. Nat Nanotechnol. 2011;6:720-725.
34. Hemphill MA, Dabiri BE, Gabriele S, Kerscher L, Franck C, Goss JA, Alford PW, Parker KK. A possible role for integrin signaling in diffuse axonal injury. PLoS ONE. 2011;6:e22899.
33. Alford PW, Dabiri BE, Goss JA, Hemphill MA, Brigham MD, Parker KK. Blast-induced phenotypic switching in cerebral vasospasm. PNAS. 2011;108:12705-12710
32. McCain ML, Parker KK. Mechanotransduction: the role of mechanical stress, myocyte shape, and cytoskeletal architecture on cardiac function. Pflugers Arch – Eur J Physiol. 2011;462:89–104.
31. Grosberg A, Kuo P-L, Guo C-L, Geisse NA, Bray M-A, Adams WJ, Sheehy SP, Parker KK. Self-organization of muscle cell structure and function. PLoS Comput Biol. 2011;7:e1001088.
30. Pong T, Adams WJ, Bray MA, Feinberg AW, Sheehy SP, Werdich AA, Parker KK. Hierarchical architecture influences calcium dynamics in engineered cardiac muscle. Exp Biol Med. 2011;236:366-373
29. Vandeparre H, Gabriele S, Brau F, Gay C, Parker KK, Damman P. Hierarchical wrinkling patterns. Soft Matter. 2010;6:5751-5756.
28. Bray MA, Adams WJ, Geisse NA, Feinberg AW, Sheehy SP, Parker KK. Nuclear morphology and deformation in engineered cardiac myocytes and tissues. Biomaterials. 2010;31:5143-5150.
27. Badrossamay MR, McIlwee HA, Goss JA, Parker KK. Nanofiber assembly by rotary jet spinning. Nanoletters. 2010;10:2257-2261.
26. Feinberg AW, Parker KK. Surface-initiated assembly of protein nanofabrics. Nanoletters. 2010;10:2184-2191.
25. Göktepe S, Abilez OJ, Parker KK, Kuhl E. A multiscale model for eccentric and concentric cardiac growth through sarcomerogenesis. J Theor Biol. 2010;265:433-442.
24. Alford PW, Feinberg AW, Sheehy SP, Parker KK. Biohybrid thin films for measuring contractility in engineered cardiovascular muscle. Biomaterials. 2010;31:3613-3621.
23. O’Grady M, Kuo P, Parker KK. Optimization of electroactive hydrogel actuators. ACS Appl Mater Interfaces. 2010;2:343-346.
22. Domian IJ, Chiravuri M, van der Meer P, Feinberg AW, Shi X, Shao Y, Wu SM, Parker KK, Chien KR. Generation of functional ventricular heart muscle from mouse ventricular progenitor Cells. Science. 2009;326(5951):426-429. [video download, 1.6 MB]
21. Geisse NA, Sheehy SP, Parker KK.Control of myocyte remodeling in vitro with engineered substrates. In Vitro Cell Dev Biol Anim. 2009;45:343-350.
20. Sheehy SP, Huang S, Parker KK. Time-warped comparison of gene expression in adaptive and maladaptive cardiac hypertrophy. Circ Cardiovasc Genet. 2009;2:116-124.
19. Bol M, Reese S, Parker KK, Kuhl K. Computational modeling of muscular thin films for cardiac repair. Computational Mechanics. 2009;43:535-544.
18. Chien KR, Domain IJ, Parker KK. Cardiogenesis and the complex biology of regenerative cardiovascular medicine. Science. 2008;322:1494-1497. Review.
17. Parker KK, Tan J, Chen CS, Tung L. Myofibrillar architecture in engineered cardiac myocytes. Circ Res. 2008;103:340-342.
16. Bray MA, Sheehy SP, Parker KK. Sarcomere alignment is regulated by myocyte shape. Cell Motil Cytoskeleton. 2008;65:641-651.
15. Geisse NA, Feinberg AW, Kuo P, Sheehy S, Bray MA, Parker KK. Micropatterning Approaches for Cardiac Biology. In: Khademhosseini A, Toner M, Borenstein JT, Takayama S, editors. Micro- and Nanoengineering of the Cell Microenvironment: Technologies and Applications. Boston: Artech House; 2008:341-357.
14. O’Grady ML, Parker KK. Dynamic control of protein-protein interactions. Langmuir. 2008;24:316-322.
13. Feinberg AW, Feigel A, Shevkoplyas SS, Sheehy S, Whitesides GM, Parker KK. Muscular thin films for building actuators and powering devices. Science. 2007;317:1366-1370. [video download, 11 MB]
12. Parker KK, Ingber DE. Extracellular matrix, mechanotransduction and structural hierarchies in heart tissue engineering. Philos Trans R Soc Lond B Biol Sci. 2007;362:1267-1279.
11. Bray MA, Geisse NA, Parker KK. Multidimensional detection and analysis of Ca2+ sparks in cardiac myocytes. Biophys J. 2007;92:4433-4443. [source code / test data]
10. Adams WJ, Pong T, Geisse NA, Sheehy S, Parker KK. Engineering design of a cardiac myocyte. J Computer-Aided Materials Design. 2007;14:19-29.
9. Brangwynne CP, MacKintosh FC, Kumar S, Geisse NA, Talbot J, Mahadevan L, Parker KK, Ingber DE, Weitz DA. Microtubules can bear enhanced compressive loads in living cells because of lateral reinforcement. J Cell Bio. 2006;173:733-741.
8. Huang S, Brangwynne CP, Parker KK, Ingber DE. Symmetry-breaking in mammalian cell cohort migration during tissue pattern formation: role of random-walk persistence. Cell Motil Cytoskeleton. 2005;61:201-213.
7. Parker KK, Lavelle JA, Taylor LK, Wang Z, Hansen DE. Stretch-induced ventricular arrhythmias during acute ischemia and reperfusion. J Appl Physiol. 2004;97:377-383.
6. Latimer DC, Roth BJ, Parker KK. Analytical model for predicting mechanotransduction effects in engineered cardiac tissue. Tissue Eng. 2003;9:283-289.
5. Bursac N, Parker KK, Iravanian S, Tung L. Cardiomyocyte cultures with controlled macroscopic anisotropy: a model for functional electrophysiological studies of cardiac muscle. Circ Res. 2002;91:e45-e54.
4. Parker KK, Brock AL, Brangwynne C, Mannix RJ, Wang N, Ostuni E, Geisse NA, Adams JC, Whitesides GM, Ingber DE. Directional control of lamellipodia extension by constraining cell shape and orienting cell tractional forces. FASEB J. 2002;16:1195-1204.
3. Parker KK, Taylor LK, Atkinson JB, Hansen DE, Wikswo JP. The effects of tubulin-binding agents on stretch-induced ventricular arrhythmias. Euro J Pharm. 2001;417:131-140.
2. Brangwynne C, Huang S, Parker KK, Ingber DE, Ostuni E. Symmetry breaking in cultured mammalian cells. In Vitro Cell Dev Bio. 2001;36:563-565.
1. Parker KK, Wikswo JP Jr. A model of the magnetic fields created by single motor unit compound action potentials in skeletal muscle. IEEE Trans Biomed Eng. 1997;44:948-957.
Congratulations to Dr. McCain!! April 28th, 2013
Congratulations to Dr. Megan McCain who has accepted a position as Assistant Professor of Biomedical Engineering at the University of Southern California! She will arrive in LA to start building her laboratory at USC in Jan 2014. Congrats to Professor McCain!!
Welcome to the DBG!! April 17th, 2013
Welcome to the DBG!! April 15, 2013
The DBG would like to welcome Roza Mahmoodian, who joins us as a postdoctoral fellow from Massachusetts Institute of Technology’s Lab for Material Chemomechanics, under the direction of Professor Krystyn J. Van Vliet. We also welcome Hyojin Ko from Sogang University in Korea as a visiting student. The DBG is excited to have both joining the group! Welcome.
Images from the DBG April 17th, 2013
Ventricular cardiomyocytes from neonatal rats can be cultured on substrates coated with extracellular matrix proteins anisotropically. These cardiomyocytes can grow according to spatial cues provided by proteins attached to a substrate. Soft-lithography allows for customizable, micron-scale protein patterns to be “stamped” onto a substrate. This allows for building tissues that more closely resemble their orzanization in vivo. This image shows cardiomyocytes aligned to 10 micron wide lines of fibronectin separated by 10 micron wide lines of fibronectin at a lower concentration.
Photo by Borna Dabiri
Disease Biophysics Group Postdoctoral Fellow Positions January 18th, 2013
The Disease Biophysics Group at Harvard University invites applications for a number of Postdoctoral Fellow positions open for projects pertaining to building microscale models of diseased organs. The project requires the assembly of engineered, human microtissues in microfluidic devices that can be interrogated for the genetic, morphological and functional indicators of health, disease, and injury.
The Disease Biophysics Group is a multidisciplinary research group based in the Harvard School of Engineering and Applied Sciences, the Wyss Institute for Biologically-Inspired Engineering, and the Harvard Stem Cell Institute.
Candidates with experience in neuronal cell biology, striated and smooth muscle tissue engineering, the cardiac valves, and experimental models of diabetes are encouraged to apply. Applicants are expected to hold a doctoral degree in engineering, physiology, or cell biology and have a proven record of high quality publications.
The application will be assembled as single pdf file: cover letter describing research interests and goals, CV, research statement, a full list of publications and up to three examples of first author papers, and a list of no less than three references with contact information. Please note that all requirements for the doctoral degree must be completed prior to the start date. The application should be sent to:firstname.lastname@example.org
Full consideration will be given to all applications received by February 15, 2013; applications received thereafter will be considered until the positions are filled.
Harvard University is an equal opportunity employer. Women and underrepresented minorities are particularly encouraged to apply.
Congratulations to Benjamin Potter and Calla Glavin for receiving Honorable Mention December 12th, 2012
The DBG congratulates Benjamin Potter and Calla Glavin for receiving Honorable Mention for their poster titled, “Effect of Polymer and Solvent Properties on Nanofiber Morphology in Rotary Jet-Spinning” at the 2012 Materials Research Society Fall Meeting Poster Competition. The poster competition was held on Monday November 26, 2012 at the Hynes Convention Center in Boston.
Both Ben and Calla were summer interns in the DBG mentored by graduate student, Holly M. Golecki. Ben and Calla are currently cadets at the United States Military Academy at West Point.