Spark Analysis Detection
Source Code / Test Data
The material here is based on the algorithm described in Mark-Anthony Bray, Nicholas A. Geisse and Kevin Kit Parker, “Multidimensional detection and analysis of Ca2+ sparks in cardiac myocytes,” Biophysical Journal, 92(12): pg 4433 – 4443 [Link].
Movie (supplemental data) – Movie recorded from an isotropic monolayer of neonatal rat cardiac myocytes, loaded with the Ca2+ sensitive dye Fluo-4. The tissue exhibits several tissue-wide calcium transients, accompanied by spark activity.
- Distribution details, including specifics of the files available (Download).
Please see the distribution file for details on the source code and the format and contents of the test data.
- MATLAB source code and GPL for spark detection (Download).
- Spark synthetic data with uniform background flourescence. (Download). ZIP file, 65.6 MB.
- Spark synthetic data with Ca2+ reuptake background flourescence. (Download). ZIP file, 65.6 MB.
This toolbox is released under the GNU General Public License (GPL, see http://www.gnu.org/licenses/gpl.html). This is a copyleft license, which means you have the freedom to use, distribute and modify the code, but only on the condition that you must pass on this freedom. You can integrate this code into proprietary packages, but you must do so according to this rule. That is, some parts of your proprietary package will not have this freedom, but those parts derived from this code must retain that freedom. You must use, distribute and develop the code herein in accordance with the GPL.
Congratulations to Karaghen Hudson, Leila Deravi & Nina Sinatra on the cover of Macromolecular Materials and Engineering! March 27th, 2017
Parker Lab Artist Karaghen Hudson’s illustration accompanying Leila Deravi & Nina Sinatra’s paper “Design and Fabrication of Fibrous Nanomaterials Using Pull Spinning” was chosen for the March 2017 cover of Macromolecular Materials and Engineering.
Pull spinning is a new nanofiber manufacturing technique that uses a high-speed rotating bristle to draw anisotropic nanofibers from a polymer solution. The versatile structure and composition of scaffolds formed using pull spinning enables a wide range of applications, including muscle tissue engineering and textile design.
Congratulations to George Touloumes! March 21st, 2017
Congratulations to Parker Lab PhD student George Touloumes who has been awarded an NSF Graduate Research Fellowship.
Congratulations to Ben Pope! March 21st, 2017
Congratulations to Parker Lab Postdoc Ben Pope who was recently awarded a Life Sciences Research Foundation Fellowship sponsored by the Good Ventures Foundation.
Congratulations Grant Gonzalez and Michael Rosnach on the cover of Macromolecular Materials and Engineering! January 23rd, 2017
Parker Lab Artist Michael Rosnach’s illustration accompanying PhD Student Grant Gonzalez’s paper “Production of synthetic, para-aramid and biopolymer nanofibers by immersion rotary jet-spinning” was chosen for the January 2017 cover of Macromolecular Materials and Engineering.
“Utilizing a precipitant vortex, a novel nanofiber platform produces Kevlar, nylon, DNA, and alginate nanofibers for high-performance composites and tissue engineering applications.”
Congratulations to Ian Perkins & Alex Cho! January 5th, 2017
The DBG would like to congratulate Ian Perkins and Alex Cho who both graduated from Northeastern University in December. Ian received his B.S. in Mechanical Engineering and Alex his B.S. in Biology. We would like to thank you both for your significant contributions to the DBG over the past several years, and we are grateful that you are both continuing with us in the lab this semester!